The Role of the Macrophage in the Innate Response of the Immune System

Developed by: Linda R. Loeffler
© American Association of Immunologists 2001

Overview
The curriculum will provide information on a scenario of a contagious or communicable disease and how it can be spread through a community. An ELISA test will be done to detect the extent of the disease and trace back to the likely origin. This lab can be used within a unit on the immune system, or may be used in a unit on health unit about specific disease, such as tuberculosis. Historically, it can be used to discuss small pox and hot it was eradicated worldwide. Of special interest to students may be HIV and AIDS as an ELISA test is used to diagnose this infection. Emerging viruses are also a hot topic lately with such newsmakers as Ebola and the West Nile viruses. There are many options in which an antigen-antibody reaction can be taught.

Objectives

 1. Students will be able to discuss and answer questions about the role of the macrophage in the innate immune response.

2. Students will be able to distinguish between an antigen and an antibody.

3. Students will be able to perform an ELISA test.

4. Students will be able to answer questions concerning the ELISA test including the use of the washes, positive and negative controls, and the use of triplicate tests. Students will also be able to discuss false-positive and false-negative test results and the significance thereof.

5. Students will be able to analyze the date from the ELISA test.